Topic of the Week

Beer's Law
Absorption spectrophotometry is the workhorse of analytical chemistry.
Measurements are quick, accurate, safe, and inexpensive. Absorption
spectrophotometry is one of the top three lab topics in general chemistry.
 
Although spectrophotometry is based on color, it is almost always
taught with instruments that present a black-and-white graphical view of a
colorful, conceptual, and interesting science.
 
MicroLab can improve this situation. Our inexpensive Visual Spectrometer makes color and absorption spectra quickly come to life. MicroLab’s FASTspecscanning spectrophotometer software will engage your students with a full color view of each sample’s molecular absorption spectra. Students transparently convert observations to graphs and mathematical models. They can use this information to accurately measure solution concentration, reaction kinetics, turbidity, scatter, and fluorescence, and to conduct spectrophotometric titrations.
 
FASTspec’s unique ability to make simultaneous measurement of transmission/ absorption spectra,
and scatter and fluorescence with multiple excitation wavelengths, encourages inquiry. Students can run a
standard Beer’s Law plot and then ask “Is there more to it?” There almost always is.
microLAB's Software
FS-528 Spectrophotometry Software
Video
Colorimetry: A Beer's Law Investigation
Spectrophotometry, sometimes called “colorimetry” is an instrumental method based on the light absorption by colored solutions and is widely used for chemical analysis. This experiment will utilize 5 crystal violet solutions of known concentration to create a Beer's Law Plot.  The concentration of an unknown solution will found using the Beer's Law Plot.
 
A similar experiment can be easily done with food color samples.

Click on graph to watch video
Click Here to Download Experiment
Equipment Used:

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